GUF leads WP5 (Constructing and evaluating optimised production strains).
GUF is represented in CHASSY by PIs from the Department of Physiology and Genetics of Lower Eukaryotes. The department has expertise in different areas of molecular biology and will participate in most scientific workpackages. Their major contributions are…
The CHASSY Team at GUF:
ORCID ID: orcid.org/0000-0002-6118-1517
Dr Oreb is project leader in the Yeast Physiology and Biotechnology Group. He has a strong background in protein biochemistry and biophysics and is an expert in engineering transporters, enzymes, and artificial protein agglomerates for applications in metabolic engineering of Saccharomyces cerevisiae.
Prof. Boles has extensively studied the central carbon metabolism of yeasts with a special emphasis on transport of metabolites across biological membranes. He brings expertise on Saccharomyces cerevisiae as well as experience in developing molecular tools, molecular biology, synthetic biology, yeast genomics and physiology, and yeasts for biotechnological applications.
Leonie uses a variety of genetic engineering tools to construct Saccharomyces cerevisiae strains with an improved production rate and high stress-tolerance. To screen for strains with these traits, she designs and develops biosensors, which can detect extracellular metabolites in a high-throughput system.
Florian is improving the production rates in Saccharomyces cerevisiae strains by metabolic engineering. He is optimizing key enzymes for higher turnover rates and product specificity and constructs pathways for optimized supply of the main metabolic precursors.